Description
Description: This assay employs a two-site sandwich ELISA to quantitate IL22RA1 in samples. An antibody specific for IL22RA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL22RA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL22RA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL22RA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: IL22R belongs to the class II cytokine receptor family, and has been shown to be a receptor for interleukin 22 (IL22). IL22 receptor is a protein complex that consists of this protein and interleukin 10 receptor, beta (IL10BR/CRFB4), a subunit also shared by the receptor complex for interleukin 10 (IL10). This gene and interleukin 28 receptor, alpha (IL28RA) form a cytokine receptor gene cluster in the chromosomal region 1p36. Expressed in colon, liver, lung, pancreas and kidney. No expression in immune cells such as monocytes, T-cells, and NK-cells. Expressed in keratinocytes of normal skin as well as in psoriatic skin lesion. Detected in normal blood brain barrier endothelial cells as well as in multiple sclerosis lesions; Strongly expressed on central nervous system vessels within inflitrated multiple sclerosis lesions